Jurkat Cells 96 Well Plate

Eb ao dye mix 8 μl was added to each well and cells were viewed under the same microscope as above.

Jurkat cells 96 well plate. Stainfree cell counts vs. 50 000 and 1562 cells seeded per well of a 96 well plate. 1 reduction of the time period during the test and the processing of the results. For both suspension and adherent cells 96 well plates were centrifuged at 1 000 rpm 129 g for 5 minutes using a beckman model tj 6 centrifuge with inserts for 96 well plates.

Prepare aliquots to avoid repeated freeze thaw cycles. Jurkat zellen die bei zelldichten von 390 bis 50 000 zellen pro well ausgesät wurden wurden mit der stainfree technologie blaue punkte analysiert oder sie wurden mit dem farbstoff des earlytox live cell assays gefärbt und die grün fluoreszierenden zellen ausgezählt grüne punkte. Incubate at 37 c for 2 hours or 4 c overnight. Tests were done in triplicate counting a minimum of 100 total cells each.

3 several conditions can be tested at the same time. Plate 200 μl of cell culture i e 50 000 200 000 cells into the wells of the sterile 96 well filter bottom plate. Back to the gibco cell culture basics homepage. For this table hela cells were used.

Fluorescent cell counts jurkat cells seeded at densities ranging from 390 to 50 000 cells per well were counted using stainfree technology blue dots or they were stained with earlytox live cell assay dye and green fluorescent cells were counted green dots. I haven t worked so much with jurkat cells so far but for the experiements we made we seeded in a 96 well plate with 50 000 200 000 cells. Storage and stability store quanti luc pouches at 20 c for 12 months. Stimulate the cells as desired.

When α cd3 coating is done wash 6 well plate 1 x 5 ml pbs then add cells to each well do not plate more than 5 ml well minimum is 2 ml. For cd4 t cells incubation is usually done at. I guess you can adjust the number by just trying it out. Die mit beiden methoden bestimmten zellzahlen stimmen über alle zelldichten hinweg nahezu überein.

For the unstimulated control wells add 50 µl of sterile pbs. Wash plate microwells 3 times with sterile pbs. This new adhesion technique has several advantages in comparison with its predecessor. Two cell densities are shown.

Aseptically decant antibody solution from the microwell plate. Dispense 50µl of the antibody solution to each microwell of the 96 well assay plate. 1 the number of cells on a confluent plate dish or flask will vary with cell type. Therefore we have developed a new method performed in a 96 well plate format adding the quantification of the non adherent viable cells with a colorimetric reaction.

4 quantification of viable. Each pouch contains everything needed to prepare 25 ml of reagent allowing the preparation of 500 wells of a 96 well plate. The filter plate is designed to retain particles while permitting the flow of liquids from the bottom of the plate. 2 the experiments can be done in a larger scale.

In the example presented below jurkat cells were stimulated with 20 μg ml anisomycin for 60 minutes at 37 c.